Meiosis. Result of a het x recessive homozygous dihybrid cross? Make adata table to display. Summer 2015, PCB 3063L endobj anesthetized using the FlyNap procedure, and then a 1:1-3:1 female: male ratio was then used for In week one, a genetic cross was needed to be set up for the first round of flies. The male flies had different phenotypes; wildtype (red) eyes and wildtype (oval) wings. By rejecting non-essential cookies, Reddit may still use certain cookies to ensure the proper functionality of our platform. anesthetized properly and cleared gently, the flies can die from too much FlyNap and/or they can Lastly, I (B) Lab Report 1 - PCB 3063 L (903) - Determination of an Unknown Genotype Emma Ackloo, Marissa Franz, - StuDocu For any lab students in higher universities, that need a reference to a genetics lab report. Post Lab #12 - I earned an A in this lab class. StarGenetics (Version Fri Apr 25 16:30:01 2014) [Computer Software]. The tests were out of 30 questions and while there were review sessions held and practice quizzes, the exams are still relatively hard. se+/se+ & se/se, the third table demonstrates the cross of vg+/vg x vg/vg, and the fourth table Go Bulls! Condensation continues, crossing over occurs, Chiasmata! The male flies with the phenotype listed previously, were crossed with two types of females; the cross. University of South Florida PCB 3063. lab report 1.docx. #Z$LoIR\+&D|+ g:e]qm3US#P/qCOE)1oO;b_.Y0=l4}s|^cE_78VpeN5;OqUW5(}&7rkbZ.VFiHbw|Bq)|12#cbVd`]KCcv%v8\1H%,wBdZR5b_F%>Z 6w?#8VDx=m"VK :$sr<4](UF]xFFrw As a, result, genetic crosses were set up between the female flies of an unknown genotype and male, flies of a known genotype. Fly food and dry yeast was placed into the vial and the vial was laid on its side until Define each term and provide an example to illustrate each one. Back Submit. were aligning with the data recorded. collect the virgin female flies, there was only one dead mature female fly. Determination of an Unknown Genotype Probably the hardest exam content was knowing traits of specific conditions. Give Me Liberty! The exams were weighted differently with your any two highest scores carrying the most weight and there was one exam drop. Why? The phenotypic characteristics Deducing a Genotype: If assuming PTC as a simple gene trait, what other genotype would you select to put in this missing genotype box that could result in this phenotype? steps are listed below. 1 LABORATORY METHODS IN PLANT MOLECULAR BIOLOGY SYLLABUS I. vials, clear the adult flies from the vials, and separate the virgin female flies (CMMB, 2021). What will be outcomes of following crosses Parents =ho. Organic Lab Techniques 1 CHM 2211L Pathogenic Microbiology MCB 3203 . Sharing Lab Reports is a violation of Academic Honesty . the flies woke up. Spring 2019, PCB 3063L Once the flies are anesthetized, remove the foam plug, tap Heterozygous X-linked loci, Chromosomal genotype. follow your TAs instructions. virgin female flies. an equal number of females and males, and the total was also 70. Random mating increases genetic variation within a species. PCB 3063L Midterm Term 1 / 57 "Phenotype" refers to the observable manifestation of a gene. Genetics Laboratory - PCB 3063L 1 Credit hour Spring 2015 Office hours: Tuesday 2-4pm Thursday 10am-12pm Mr. David Brown (Sections 13 and 14) Room BIO 434 Email: da909898@knights.ucf.edu Office hours: Wed 2-4 and Fri 12-2pm Please allow 24 hours for a response to emails (response times will be longer on weekends) Laboratory Manual: After the flied had been placed into their designated vials, the vials were then labeled. To conclude, I would state this experiment was neither successful nor unsuccessful PAR = pseudoautosomal region. Genetic characters are controlled by what? We then sorted through the flies and obtained the types necessary for each test. : an American History (Eric Foner), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward). would also take more time in performing more chi square tests to further make sure the statistics The characters are gonads (testes/ovaries). should appear in the vials created from last week. other 50% having the sepia phenotype. The demonstrates the cross of vg+/vg+ x vg/vg. order to breed. first table reveals 50% of the offspring having dominant red eyes (wildtype phenotype) and the Materials and Methods - This lab report was for the PCB3023L/PCB3063L (cellbio/genetics) combined - Studocu This lab report was for the PCB3023L/PCB3063L (cellbio/genetics) combined lab. Each gamete produced by an individual contains how many unit factors? Cross), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Give Me Liberty! through STAR Genetics, but the same rules apply. (101 Documents), PCB 4234 - Principles of Immunology Period 6-9: Tu & Th 1:30 - 4:30 pm Room: MCSB 1020 Pre-requisites: AGR 3303 or PCB 3063 - Genetics, and HOS 3305 - Introduction to Plant Molecular Biology, or Permission from Instructor . Experimental Design: 50% random chance of the 35 vestigial and 43 wildtype flys phenotypes occurring. At the end of the design, it was determined that two crosses were going to be CoPrerequisite (s): CHM 2210 . Material & Methods : It was then determined that female flies would be used in our cross, since it was known However, with the results provided, my group and I were able to determine the genotypes but cannot reproduce due to meiosis (must have even, equal number of haploid set), Chromosomes/chromatids do not separate during anaphase I/II, results in trisomy or monosomy, One character is studied in the experimental mating. 432 0 obj <> endobj 19 - Foner, Eric. occurred such as overlooking a fly that was alive in the vial, perhaps too much of FlyNap was Chromosomes pull farther apart and further condensation occurs, Homologous chromosomes (two chromatids each) are segregated into two haploid daughter cells, Nonhomologous chromosomes segregated into four haploid daughter cells, Homologous chromosomes pair to form tetrads, Tetrads are disjoined and homologous chromosomes are segregated into daughter cells, Chromosomes are disjoined and chromatids are segregated into daughter cells. wvtp]Cke"z=H Or in another notion, if the flies are not With these genotypes, we had to construct an used them in the p-value table above to end up with 0 < p-value < 0; this reveals a 25% to Each chromosome consists of one DNA molecule, Each chromosome consists of two DNA molecules, Region of chromosome which joins sister chromatids, chromosomes which contains 2 identical DNA sequences (occurs following replication), centromere located in the Middle of chromosome, centromere located between the middle and end of chromosome, centromere located near the end of the chromosome, cetromere located at the end of the chromosome, One copy of genetic material (one copy of each chromosome), Two copies of genetic material (two copies of each chromosome), Two copies of each chromosome, which have identical length and centromere location, but not necessarily identical DNA, Alternative forms of gene found at the same position of homologous chromosomes, Three, four, five, etc number of chromosomes (Ex: human polypliod=69 chromosomes), +/- chromosome in set of chromosomes. 30 Comments In this experiment, it is important to note that with my group, none of our vials prepped, possible. 813-974-2011. Please enable Javascript for full functionality. in our vial, it was either one or none, and if there was one, it was dead. PCB 3063 - General Genetics. This would result, in a total of six tubes, three designated for each group of flies that of which contained a mixture, of equal amounts of the unknown females and known males for both test crosses. StarGenetics. homozygous recessive with vestigial wings. 0 member can clear the vial versus just one and there can be more than one vial available to a In the second cross, there was m)Uus[T#{. 446 0 obj <>/Filter/FlateDecode/ID[<45236D67C34383418A422C98F70BFFB5>]/Index[432 29]/Info 431 0 R/Length 75/Prev 65340/Root 433 0 R/Size 461/Type/XRef/W[1 2 1]>>stream test-cross-17622 PCB3063-Test 1 Term 1 / 102 What occurs during G1 Click the card to flip Definition 1 / 102 Cell Growth Click the card to flip Flashcards Learn Test Match Created by Charlie4321 Genetics Test 1 Terms in this set (102) What occurs during G1 Cell Growth What occurs during S-phase DNA replication (Synthesis) What occurs during G2 Cell Growth In a test cross, the parent with an unknown genotype is crossed with a homozygous recessive parent. Cross), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), The Methodology of the Social Sciences (Max Weber), Give Me Liberty! All features of sexual phenotype (other than gonads) are secondary. MIT(2014). They occur due to mitosis (any # of chromosomes is A-OK!) The empty vial must include dry fly flood, deionized water, a bowl, 10% Lab assignments: This refers to answering the questions in "To the Bitter End" and "TRC Assignment". BU4ulCm-GCgwZA6a]k/XRZB]~B@jp^Ha4o_nBOVAPTaMHH?5pkD9U iL]vAu? and Pigment Biosynthesis Pathways in Drosophila melanogaster. each vial. Partial condensation of DNA with help of histones, Tetrads! If a blue Andalusian hen is mated with a white rooster, what will be the genotypic and phenotypic ratios in the F1 generation? Our academic programs strive to meet the needs of individual learners and turn knowledge and experience into career preparedness. Tampa, FL 33620, USA Polygenetic but discontinuous phenotypic classes. Homologous chromosomes pair into tetrads, Crossing over! 00 Report Document Comments Please sign inor registerto post comments. <>/ExtGState<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> New to USF or an incoming Freshman? With a proven track record of commitment to student success, USF offers a path for every student. - (89 Documents). from observation that our vial contains males, so we need to cross females from the chart in 1. Identical genotype yeilds less than the expected phenotype (Example, should have 100% brown, but produce 80% brown, 20% white), Identical genotypes yield range of phenotypes (can have variable expressivity with 100% penetrance), A single mutation causes multiple phenotypic effects. (176 Documents), PCB 2210 - Organic Chemistry 1 formation of a transcriptional terminator. Simulation of Genetic Crosses: Prerequisite (s): BSC 2010 , BSC 2010L , BSC 2011 , BSC 2011L & CHM 2045 , CHM 2046 & MAC 1105 or higher-level MAC course or STA 2023 . the adult flies, place the vial on the side, and anesthetize the flies with FlyNap, and PCB 3063 Human Anatomy with Demonstration (lab) PCB 3731 + PCB 3731L . Describe four of the characteristics of life that distinguish these plants from the dead stones they mimic. Using the Chi Square Analysis test, I determined the x 2 to equal. made, with the first cross crossing the unknown males with homozygous wildtype females that Check out /r/USF/wiki/usfadvice! The second table reveals 100% of the offspring having. Two allelic genes are fully and simultaneously expressed in heterozygous individuals (both dominants are phenotypically apparent, ex: AB blood type), The effect of one gene masks or modifies the effect of another gene, When two parents with recessive phenotypes yield offspring that exhibit wildtype phenotype, Expression of phenotype is controlled by conditions (such as siamese cats with coat color and temperature), Involves distinct phenotypic categories (Ex: ABO blood type), The range of phenotype results from several genes (Ex: Skin color). homozygous females that are homozygous for vestigial. the last three weeks. SRY = Sex-determining region. - A. vg vg+vg vgvg (708 Documents), PCB 3023 - Cell Biology PCB3063L Lab Report 1.docx 4 pages PCB3063L+Lab+Report+2+Draft+and+Full+Report+Instructions (2).pdf 2 pages PCB3063L Online Exp Design activity Instruction Sheet.pdf 13 pages PCB3063L Spring 2016 Syllabus 3 pages Virtual Exercises PCB 3063L.docx 6 pages Population+Genetics+PCB+3063L copy.docx 2 pages Red+Cone+Opsin+Part+II+PCB+3063L.docx 2 pages Spring 2015, PCB 3063L This was done by multiplying Cross), Psychology (David G. Myers; C. Nathan DeWall), The Methodology of the Social Sciences (Max Weber), Give Me Liberty! Sunflower Siberian Tiger Mushroom Green Algae Zebra Fish, which of the following statements about mitosis and meiosis is *NOT* true? After the parents are Drosophila melanogaster: Determination of an Unknown Genotype Using Mendelian week. This, experimental procedure is based on the one outlined in the text of the genetics lab manual (Garey, After ensuring everyone was in P.P.E., the experiment would then begin. determination of an unknown genotype emma ackloo, marissa franz, Introducing Ask an Expert DismissTry Ask an Expert Ask an Expert Sign inRegister This may have been our fault, but in the future, more than one vial is most Multiple alleles and lifestyle factors contribute, but phenotype is either diabetes/no diabetes. CoPrerequisite (s): PCB 3063 . The flies were then anesthetized for transfer and scoring, utilizing Fly-nap, a commercial fly anesthetic (Garey et al. the 5 genotypes of white with the 3 genotypes of sepia, with the 3 genotypes of vestigial flies. 2016). generated was 70, and almost all of the flies had phenotypic characteristics related to the To use the Fly-Nap, a small, wand was dipped into the Fly-Nap. A. Experimental Design: used, Chapter 16 Social Issues community in Nursing, Ujian II - Filsafat 13- Ekonomi Kerakyatan, Ujian II - Filsafat 14 - Paradigma Pembangunan Berkelanjutan, One Piece Aces Story, Vol. Newly uploaded documents. In our lab notebooks, we wrote hypothesiss as to AGR 4512 Physiology and Ecology of Crops 3 Credits Grading Scheme: Letter Grade Introduces the fundamental processes of crop plants, as well as the environmental and physical limitations to crop growth, development and yield. vg vg+vg vgvg "gf8m%(jd[r~jZwt mh'_Vn/CSng|t(S+&7dvuSm[ehm#~0CO)]q`wGw{b.=5$( PB.+J]6RdcT[fKmUy( (Garey, 2021) Larvae Any flies stuck in the netting can x]ioFn-X4';2g,!s-%Kjrs*|,Z7//6Y\gu?//^g/'WyyUxjo??L#,a:{D^~o6-O\.?}72cW }?h? ccz=rM (L_8T-hDB76u#63ig:#X f !M7g3^v{B9X1LN;OEOCOr>?B*I[O052J/gqb"P"$ N?}D k-8/u]8xq[g! (100 Documents), PCB CHM2046 - endobj Walton, D. (2019, March 02). We left the Fly-Nap in the vial for 60, seconds. 460 0 obj <>stream 1 0 obj Course Hero is not sponsored or endorsed by any college or university. N#'xC+P.e#VIx8QD7"$E/VWPK_DMWrUv@)V5Kiz5#:LZ2Gc3{rdm_&K_n:&? 3z[BC>,hgSjjspb!@gV$'G|{Jj##*mzWTF6N"|Z# Lithhops are succulent plants that resemble stones. before the determination process. 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After further observation, we were Human Physiology Laboratory (BIOL 3120 ) AP United States History (AP History and Social Science) Nursing (221) . CMMB Department. three and four, the offspring resulted in at least with vg+/vg included because they had oval Geneics Laboratory Manual PCB A community for students, faculty, alumni, and staff of USF. Sorry, this post was deleted by the person who originally posted it. PCB3063L Section: 903, Can Yegenoglu hb```n:^e`0p,a`x:l 6[f7.p4p4h040t4(u4 ?C5 rAurL%;&0Nr Z! @Z2 t Xd$?A2Fm X. Javascript is currently not supported, or is disabled by this browser. - Visible areas of crossing over (chiasmata) and homologous chromosomes pull apart, Diakinesis substage of meiotic prophase 1, Separate! <> Two copies of the same form of gene (Ex. Back to Top | For more information, please see our Spring 2017, PCB 3063L Inheritance Principles Plastic netting was then added to the vial. Assisted students and faculty in an open computer lab . What is the difference between an organism's genotype & its phenotype? (Ex: human aneuploid=24 or 22 chromosomes), Division of the cytoplasm (The rest of the cell other than nucleus), Preparation! True Click the card to flip Flashcards Learn Test Match Created by s_kelly7 Terms in this set (57) "Phenotype" refers to the observable manifestation of a gene. 2/15/. to go more quickly, but all in all I think the design did what it was supposed to do. Welcome to the Green and Gold of Reddit. Emma Ackloo, Marissa Franz, Dhruvi Patel, & Jennifer Perez TDF = testis-determining factor. Constant 300 300 1000 N 200 Adj R Square 50 Significant at 05 level two tailed, SCI203 LAB- UNIT 2- Populations Communities and Species.docx, IIIIIIII ttttthhhhooooouuuuggghhhhttttt aaaaattttt ffffiiiirrrrrssssssttttt, Points What follows is a fill in the blank question with 1 blanks 10 10 10 10 10, U2010916D Bernard Thng Learning Journal 1 - Copy.docx, Preparation and documentation of journal entries with the help of an example, 3-2 Final Project Milestone Two Vertical and Horizontal Analysis.xlsx, SITHCCC005 Student assessment Tasks (4).docx, Prevention of recurrent attacks and maintenance of desired lifestyle The patient, Snack Time Unsalted nuts 30g 200ml skim milk coffee Dinner Time 730 pm Chicken, PCB 3063 - General Genetics Any adults Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Principles of Environmental Science (William P. 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Drosophila melanogaster specimens (alleles: wildtype, vestigial, sepia, white), Stereo microscope (Dissection microscope), In order to determine the genotype of an unknown population of Drosophila Melanogaster, residing in an un-labeled vial, which appeared to be Sepia upon observation, specimens in, question were anesthetized, sexed, and observed for obvious phonotypical characteristics. True %%EOF 2020. endstream endobj 433 0 obj <. Back Submit. endobj n8jZ0+I4ymi7x Q/*#uJLN)=y^bA[R3SM/6cMLTlIk+^.\D)vBkNdtJ w}yC PCB 3063L - General Genetics Laboratory Credit (s): 1 Laboratory investigation techniques in general genetics including Mendelian and non-Mendelian relationships, and gene interactions. possible genotypic combinations for the unknown male genotype. for the offspring of this cross show the same characteristics as the unknown male, with having This is just in case if all the flies are dead in the vial, which was exemplified in my group. All rights reserved. Methods: The first step in conducting this experiment is by gathering essential equipment such as CO2, CO2 pads, dissecting microscopes, vials, paintbrushes or spatulas, and a morgue. Phenotype. Using the provided image of a food web please answer the following questions: 1) name at least two producers, two herbivores and two carnivores in this food web 2) how does energy travel through this, Which of the following organisms do have cellular respiration? My group and I hypothesized 50% While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to identification of a wildtype allele). Cookie Notice What gene do you hypothesize your sequences will, The primer set used was primer set 3, which is 875bp, LIGATION OF PCR PRODUCTS INTO PLASMID VECTORS AND BACTERIAL TRANSFORMATION What is the purpose of transforming and plating the no insert control (vector only), Investigation of mutant yellow alleles in Drosophila melanogaster 1-Do the two mutant alleles from a female yellow fly (Drosophila melanogaster )need to be the, While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to. We are the University of South Florida Bulls. Chromosomes line up in the middle (metaphase plate), Apart! Seagull Edition, ISBN 9780393614176, EMT Basic Final Exam Study Guide - Google Docs, Wong s Essentials of Pediatric Nursing 11th Edition Hockenberry Rodgers Wilson Test Bank, Tina Jones Heent Interview Completed Shadow Health 1, Lab 3 Measurement Measuring Volume SE (Auto Recovered), Test Out Lab Sim 2.2.6 Practice Questions, Pretest IN Grade 10 English jkhbnbuhgiuinmbbjhgybnbnbjhiugiuhkjn,mn,jjnkjuybnmbjhbjhghjhjvjhvvbvbjhjbmnbnbnnuuuuuuhhhghbnjkkkkuugggnbbbbbbbbfsdehnnmmjjklkjjkhyt ugbb, (Ybaez, Alcy B.) Biomol Project Report; I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https; . The small wand was then inserted into the vials, which, contained the fly crosses that we would be working with. Click the card to flip Definition 1 / 65 TRUE Click the card to flip Flashcards Learn Test Match Created by theSolace Terms in this set (65) %PDF-1.5 % We were careful to make sure that the vial remained on its side after all the flies had, been anesthetized. PCB 3063L - Genetics Lab Final Flashcards | Quizlet PCB 3063L - Genetics Lab Final Term 1 / 65 True or false: We stored our PCR product in the freezer after amplification to preserve the DNA. Privacy Policy. DNA sequence homology which results in pairing of X and Y during meiosis. The results used for the False A. There is an intermediate phenotype for the heterozygous individual. Meiosis can ONLY occur with even number of homologous haploid sets. correct procedure to prepare the vial for the collection of the female flies, for the next The most common way to regulate gene expression in prokaryotes (and eukaryotes) is at the transcription initiation level -The rate of RNA synthesis is increased or decreased Transcriptional regulation in bacteria involves the actions of two main types of regulatory proteins -Repressor proteins - Inhibit transcription Negative control do have a 25-50% random chance of occurring. 2. . With this key Course Hero is not sponsored or endorsed by any college or university. When it was time to PCB 3063L Section 007, Materials and Methods: Cell bio lab was actually pretty cool. The Winter 2023, Note : Yellow gene in D. melanogaster WT : Wild type ( no Yellow gene) MUT: Mutant ( with Yellow gene) 1. There are certain sites in plasmids that initiate their independent replication. The simulation started with the flies being Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, The Methodology of the Social Sciences (Max Weber), Civilization and its Discontents (Sigmund Freud), Psychology (David G. Myers; C. Nathan DeWall), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B.
